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A pap smear (also called Pap test and smear test) is a medical screening test for detecting pre-cancerous changes (termed dyskaryosis derived from Greek, meaning 'bad-nut') and thus aims to prevent the development of cancer and other abnormalities in the female genital tract by sampling cells from the cervix with an Aylesbury spatula or (more frequently with the advent of liquid-based cytology) a plastic-fronded broom. The cells are placed on a glass slide and checked for abnormalities in the laboratory. The test is simple and effective.

Since the mid-1990s, techniques based around placing the sample into a vial containing a liquid medium which preserves the cells have been increasingly used. The media are primarily ethanol based. Two of the types are Sure-Path (Medical Solutions) and Thin-Prep (Cytyc Corp). Once placed into the vial, it is usually sent to the laboratory. There, the sample is processed at the laboratory and placed onto a glass slide there.

The sample is stained using the Papanicolaou technique, in which tinctorial dyes and acids are selectively retained by cells. The aim of the staining technique is to aid identification of the abnormal or dyskaryotic cells by them staining differently to the benign cells.

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