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The testicles or testes (singular testicle), are the male generative glands in animals. Male mammals have two testicles, which are often contained within an extension of the abdomen called the scrotum.

In mammals, the testes are located outside of the body, as they are suspended by the spermatic cord and within the scrotum. This is due to the fact that spermatogenesis in mammals is more efficient at a temperature somewhat less than the core body temperature (37°C or 98.6°F for humans). The cremasteric muscle is part of the spermatic cord. When this muscle contracts, the cord is shortened and the testicle is moved closer up toward the body, which provides slightly more warmth to maintain optimal testicular temperature. When the temperature needs to be lowered, the cremasteric muscle relaxes and the testicle is lowered away from the warm body and are able to cool. This phenomenon is known as the cremasteric reflex. It also occurs in response to stress (the testicles rise up toward the body in an evolutionary effort to protect them in a fight) and they also retract during orgasm.

Animals other than mammals do not have externalized testicles. Birds, despite having very high core body temperatures have internal testes: it was once theorized that birds used their air sacs to cool the testes internally, but later studies revealed that birds' testes function at core body temperature.*

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