http://www.gourt.com/Health/Medicine/Medical-Specialties/Pathology.html en-us Copyright 2007, Gourt.com 2009-11-07T23:50+04:00 rtruog@gourt.com rtruog@gourt.com Pathology RSS : Gourt hourly 1 1901-01-01T00:00+00:00 http://www.physemp.com/physician_jobs/all_pathology_jobs_in_minnesota/page_1.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_arizona/page_2.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_pennsylvania/page_2.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_pennsylvania/page_1.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_north_carolina/page_2.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_mississippi/page_2.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_south_carolina/page_2.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_mississippi/page_1.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_new_mexico/page_1.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_new_york/page_1.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_north_carolina/page_1.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_florida/page_1.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_maryland/page_1.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_south_carolina/page_1.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_louisiana/page_1.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_georgia/page_1.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_maryland/page_3.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_north_dakota/page_2.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_virginia/page_3.html http://www.physemp.com/physician_jobs/all_pathology_jobs_in_texas/page_2.html http://www.ann-clinmicrob.com/content/8/1/29 160 ug/ml). Optical and electron microscopy experiments revealed relevant alterations in cell shape and size in all strains treated with 1 and 2.5 ug/ml of subfraction F2.4. Capsule size of the capsular strains decreased drastically after subfraction F2.4 treatment. In addition, ultrastructural alterations such as cell wall disruption, cytoplasm extraction, mitochondria swelling, increase in the number of cytoplasmic vacuoles and formation of membranous structures in the cytoplasm were also observed in treated yeasts. Incubation with subfraction F2.4 also decreased C. neoformans pigmentation, however, did not interfere in melanization of B16F10 mammalian cells. Conclusion: Our data indicate that tannins extracted from S. adstringens interfered with growth, capsule size and pigmentation, all important virulence factors of C. neoformans, and may be considered as a putative candidate for the development of new antifungal agents.]]> http://www.ann-clinmicrob.com/content/8/1/28 128 μg/ml (most >2048 μg/ml), lysostaphin and Conflikt®, with relatively low MBICs, were used to correlate Alamar blue reduction with 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) reduction and viable counts (CFU/ml) for S. aureus ATCC 29213 and three clinical isolates. Alamar blue's stability and lack of toxicity allowed CFU/ml to be determined from the same wells as Alamar blue absorbances. Results: Overall, Alamar blue reduction had excellent correlation with XTT reduction and with CFU/ml. For ATCC 29213 and two clinical isolates treated with lysostaphin or Conflikt®, Alamar blue reduction had excellent correlation with XTT reduction (r = 0.93-0.99) and with CFU/ml (r = 0.92-0.98). For one of the clinical isolates, the results were moderately correlated for Conflikt® (r = 0.76, Alamar blue vs. XTT; r = 0.81, Alamar blue vs. CFU/ml) and had excellent correlation for lysostaphin (r = 0.95, Alamar blue vs. XTT; r = 0.97, Alamar blue vs. CFU/ml). Conclusion: A reliable, reproducible method for evaluating biofilm susceptibility was successfully applied to S. aureus biofilms. The described method provides researchers with a simple, nontoxic, relatively inexpensive, high throughput measure of viability after drug treatment. A standardized biofilm Alamar blue assay should greatly increase the rate of discovery of S. aureus biofilm specific agents.]]> http://www.ann-clinmicrob.com/content/8/1/27 http://www.ann-clinmicrob.com/content/8/1/26 http://www.ann-clinmicrob.com/content/8/1/25 http://www.ann-clinmicrob.com/content/8/1/24 http://www.ann-clinmicrob.com/content/8/1/23 http://www.ann-clinmicrob.com/content/8/1/22 http://www.ann-clinmicrob.com/content/8/1/21 79.5%) and comprised of thirteen unique pulsotypes, with genotype VII circulating in both hospitals. The presence of blaOXA-23 in 13% (11/83) and ISAba1 linked blaOXA-66 in 79.5% (66/83) of clinical isolates was associated with high level imipenem resistance. In this set of OXA producing isolates, multidrug resistance was bestowed by blaADC-25, class 1 integron-borne aminoglycoside modifying enzymes, presence of sense mutations in gyrA/parC and involvement of active efflux (with evidence for the presence of adeB efflux gene). Conclusion: This study underscores the major role of carbapenem-hydrolyzing class D β-lactamases, and in particular the acquired OXA-23, in the dissemination of imipenem-resistant A. baumannii. The co-occurrence of additional resistance determinant could also be a significant threat.]]> http://www.ann-clinmicrob.com/content/8/1/20